intraperitoneal injection in humans

However, no significant TSG-6 difference was found between AI and IV groups. An official website of the United States government. Article Article In another Phase I clinical trial, patients with ovarian cancer were injected intraperitoneally with dl1520 in order to study the effects of a replication-competent/-selective virus. Many published studies have showed the benefits of IV delivery in IBD treatment. Best way for mesenchymal stem cells transplantation for colitis. 2020 Jun 3;37(6):119. doi: 10.1007/s11095-020-02818-8. The good cell-cell cross talk between MSCs and immune cells may be another contributor for better therapeutic effects. Gastroenterology 140, 17851794, doi: 10.1053/j.gastro.2011.01.055 (2011). 2018 Jul;6(3):207-216. doi: 10.29252/beat-060304. Solid lipid nanoparticle delivery of rhynchophylline enhanced the efficiency of allergic asthma treatment via the upregulation of suppressor of cytokine signaling 1 by repressing the p38 signaling pathway. Intraperitoneal Drug Administration - an overview | ScienceDirect Topics The measurement of inflamed colon length demonstrated therapeutic effects of MSCs injection. The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Colitis showed varying degrees of alleviation after three ways of MSCs transplantation and the IP injection showed the highest survival rate of 87.5% and displayed the less weight loss and quick weight gain. It was concluded that there is evidence that IP may not be the best method of euthenasia due to possibilities of missinjection. The IP and AI groups showed mean 50.4% and 46.7% Ki-67+ cells/crypt respectively. A . [2] Killing the rodent through an intraperitoneal route was originally recommended over other routes such as inhalants because it was thought to be more efficient and ethical. (A) Representative IHC images of each treatment group. Gastroenterology 132, 944954, doi: 10.1053/j.gastro.2006.12.029 (2007). Afterwards, the samples were incubated with Ki-67 (1:200; Santa Cruz) or FOXP3 (1:100; Antibodies-Online, Aachen, Germany) overnight at 4C. The dynamic in vivo distribution of bone marrow-derived mesenchymal stem cells after infusion. Adv Exp Med Biol 371A, 6165 (1995). Cancer Biother Radiopharm 28, 335342, doi: 10.1089/cbr.2012.1351 (2013). & Ramesh, T. Comparative investigation of the differentiation capability of bone-marrow- and adipose-derived mesenchymal stem cells by qualitative and quantitative analysis. local inflammation and granulomatous reactions at the site of injection, lymph node structural changes, chronic inflammation, skin ulceration, local abscess or tissue sloughing, diffuse systemic . Currently, therapy still largely relies on empirical and often implemented in a stepwise fashion: progressing through 5-aminosalicylate compounds, corticosteroids, immunomodulatory drugs and finally anti-TNF drugs7. CAS Humans Injections, Intraperitoneal Peritoneal Cavity* / blood supply Peritoneal Neoplasms / drug therapy Peritonitis / drug therapy Substances Anti-Bacterial Agents Antineoplastic Agents . Here, we combined the iDISCO + method and Fos immunostaining to confirm that the middle part of the right-side, but not the left-side, insular cortex in adult male mice is activated by intraperitoneal injection of lithium chloride. During the observation, the body weight changes and fecal blood and their survival date was faithfully record. However, if proper placement of the needle occurs, this is not necessary. Intraperitoneal Injection - an overview | ScienceDirect Topics Blood 109, 46274634, doi: 10.1182/blood-2006-12-064345 (2007). PubMed We notice that the mean density of collagen was lowest in IP group, however, IP group did not exhibit less collagen deposition than the AI group, probably due to the scatter points (Fig. Here, three different MSCs delivery routes: intraperitoneal (IP), intravenous (IV) and anal injection (AI) were compared on DSS-induced colitic mice model. Influence of Systemic Administration of Coq10 Nanoparticles on Ischemia-Reperfusion Injury on Ovaries in Rat. These findings may benefit clinic practice. If injecting a hazardous substance (biohazard, radiation hazard or chemical hazard), include precautions in the Animal Care Protocol for human safety (e.g. Background. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. The overall therapeutic factors, MSCs migration and targeting as well as local immunomodulatory cytokines and FoxP3+ cells infiltration were analyzed. Every 1107 cells were incubated with 10mL DiR solution (PBS based containing 3.5g/mL dye and 0.5% ethanol) for 30min at 37C. In study by Pennesi et al., MSCs was found to be able to prevent damages caused by collagen-induced arthritis, despite of lack of a detectable presence in the arthritic joint45. Little is known about neuronal cell death in mouse models of diabetic retinopathy. Intraperitoneal injection. The excitation/ emission spectrum of DiR is in the near infrared range (excitation 750nm and emission 782nm). The three groups can decrease the mortality rate compared with PBS control, however, the IP injection showed the highest survival rate of 87.5% (P=0.0021vs. Control, Fig. [7] In 1969, errors depending on a variety of techniques of administering IP injections were analyzed, and a 12% error in placement was found when using a one-man procedure versus a 1.2% error when using a two-man procedure. [11] The effects of this study were the onset of flu-like symptoms, emesis, and abdominal pain. Intraperitoneal (IP) Injection (Injection of Sodium Pentobarbital . MSCs were isolated from C57BL/6 mice bone marrow as previously reported62. All reactions were performed in triplicated. Because it allows administering a larger volume with more radioactivity, we have investigated this route and the possibility of using it to circumvent the volume constraint we earlier experienced with pretargeting radiotherapy. Infiltration (I): 0, no infiltrate; 1, infiltrate around crypt basis; 2, infiltrate reaching to lamina muscularis mucosa layer; 3, extensive infiltration reaching the muscularis mucosa with abundant edema; 4, infiltration of the submucosa layer. Injection into egg 1 month 20 doses 1 dose 1 month 1 month Once 3.0 mL/kg 5 mL/kg 10 mL/kg 2.5 mL/kg 1 mL/kg O.^L/g Well tolerated Well tolerated Well tolerated Well tolerated Well tolerated Less mortality 1 \Jg Increase in mortality, decreased activity during righting reflex, running time, visual discrimination, and olfactory aversion test and arterial blood in humans during peritoneal dialysis therapy with 4 mmol 5-fluorouracil. Intraperitoneal injection is widely used in rodents as a route of drug administration. Collagen Deposition was assessed with phosphomolibidic acid-picrosirius red staining. Stem cell reviews 10, 127144, doi: 10.1007/s12015-013-9468-x (2014). 2021 Jul 23;2021:2303417. doi: 10.1155/2021/2303417. Adhesions are a leading cause of secondary infertility in women, accounting for 15% to 20% of cases. 4D,E). Google Scholar. Federal government websites often end in .gov or .mil. The provided examples show a variety of uses for intraperitoneal injections in animals for in vitro studies. Though genetic and environmental factors are believed to be involved in the disease, not much progress has been made on therapy. TSG-6 might be a major anti-inflammatory mechanism of MSCs, since not only in colitis model, the therapeutic effect of TSG-6 remotely secreted by MSCs were also identified in many other disease model46,48,49,50. Intraperitoneal injections as an alternative method for micro-CT B.X. It was determined that IP injections are a good option for Fenestra to quantify liver tumors in mice. Department of Digestive Diseases, State Key Laboratory of Cancer Biology, Xijing Hospital, Fourth Military Medical University, Xian, 710032, China, Min Wang,Cong Liang,Hao Hu,Lin Zhou,Bing Xu,Xin Wang,Ying Han,Yongzhan Nie,Jie Liang&Kaichun Wu, Department of Gastroenterology, Xian Childrens Hospital, 710006, China, Department of Respiratory and Gastroenterology, Second Peoples Hospital, Xian, 710005, China, Department of Gastroenterology, PLA No.5 Hospital, Yinchuan, 750004, China, You can also search for this author in Samples were centrifuged at 30,000g for 20minutes and stored at 80C until cytokine determination. Mouse insulin gradually decreased and was no more detectable 13 weeks after STZ injection. PubMed ADS Histological evaluation was completed in a double-blind fashion by two independent pathologist. gynecological and gastrointestinal cancers and has shown very promising results. Muller-Ehmsen, J. Stem cells and development 18, 683692, doi: 10.1089/scd.2008.0253 (2009). Growth hormone (B) Quantification of % Ki-67+ cells/crypt, the IP and IA>IV>DSS control, n=6 mice/group; n=3 for the control group. Supplies: Sminia, T., Soesatyo, M., Ghufron, M. & Thepen, T. The migration of peritoneal cells towards the gut. Available at: http://www.cdc.gov/ibd/ (Accessed: 7th April 2016) (2014). Surg Clin North Am 87, 727741, doi: 10.1016/j.suc.2007.03.004 (2007). Ankrum, J. Khalil, P. N. et al. These time-tested therapies may perform well on some patients, but also exhibit inadequacies in efficacy. Gynecol Oncol 81, 7781, doi: 10.1006/gyno.2000.6108 (2001). Intraperitoneal injections are typically made in the lower right or left quadrant of the hamster's abdomen, not on the midline. Cell and tissue research 347, 419427, doi: 10.1007/s00441-011-1306-3 (2012). Clipboard, Search History, and several other advanced features are temporarily unavailable. (D) Semi-quantitative fecal occult blood test on day 3. You are using a browser version with limited support for CSS. CBSKL2015Z01 and CBSKL2015Z12), PLA 12th Five-Year Program (BSW11J013) and National Science and Technology Major Project (No. A modified human recombinant type 5 adenovirus . After centrifuge, the cells were certificated and transferred to MSC expansion culture medium consisting of a-minimum essential medium (a-MEM; Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 100g/mL penicillin/streptomycin. Yu-Lung Chang, Huei-Yu Lo, Chin-Kan Chan, Xing-hua Pan, Qing-qing Li, Guang-ping Ruan, Hongsheng Yang, Rui Feng, Shenghong Zhang, H. R. Wardill, C. E. M. de Mooij, N. M. A. Blijlevens, Suze A. Jansen, Edward E. S. Nieuwenhuis, Caroline A. Lindemans, Scientific Reports And the loss of MSCs in the colon may attribute to many reasons, such as, wash out, cell death, or even rejection via the innate immune system. PDF Proper Method for Intraperitoneal Injections - PerkinElmer Int J Stem Cells 1, 17 (2008). After wishing with PBS, the sections were incubated 1h at room temperature with secondary antibody, then visualized by diaminobenzidine (DAB). The authors give their special thanks to the Dr. Yingchun Jia and Zhen Chen for their help in histological evaluation. DSS only served as control. 2020 May 9;12(5):439. doi: 10.3390/pharmaceutics12050439. Cancer cell 23, 107120, doi: 10.1016/j.ccr.2012.11.013 (2013). Course of Crohns disease after allogeneic marrow transplantation. Nature biotechnology, doi: 10.1038/nbt.2816 (2014). 17 The influence of the sex of the animal on anesthetic agents remains poorly researched and understood. Half of the volume of your D-luciferin substrate can be injected on one side of the animal and the other half of the volume on the opposite side. Rep. 6, 30696; doi: 10.1038/srep30696 (2016). Sphingosine-1-phosphate links persistent STAT3 activation, chronic intestinal inflammation and development of colitis-associated cancer. Colonic mRNA expression of inflammatory mediators such as TNF-, IL-6, IL-1, IL-10 and IFN- were analyzed by real time-PCR in order to evaluate the local inflammatory environment (Fig). Overall, this section provides a few examples of the effects and uses of intraperitoneal injections in human patients. At 72h, the IP groups showed significant higher level of TSG-6 than AI groups and DSS control (P=0.023 and P=0.005, respectively). The accessibility of the therapeutic cells to their expectable target site is strongly dependent on the route of administration used. Horses and other large animals: 1 mL per 10 lb of body weight, to a maximum . Journal of biomedical science 15, 585594, doi: 10.1007/s11373-008-9256-9 (2008). Side effects. Influence of Aggregation and Route of Injection on the - PLOS It is more often applied to animals than to humans. PDF Intraperitoneal (IP) Injection in Rats and Mice SOP Once the animal is properly restrained (as described above for SC injections) the injection site is . The increasing evident indicated that cell-cell contact between MSCs and immune cells may also be of importance52,53,54. Sirius red staining showed disorganized collagen fibers in DSS control colon, which were diffusely distributed in the injured colon wall. The fecal occult blood test on the day 3 also showed nearly complete absence of occult blood in IP group (Figure 1D). The AI groups exhibited 9.5% and IV group showed 6.7% FoxP3+ cells, respectively (Fig. Our work used marrow-derived MSCs but not adipose-derived ones. In IP group, the MSCs existence can be detected at day 1, but gradually decreased with the time, GFP+ cells can be hardly identified in inflamed colon after day 3 (data not shown). Cell stem cell 5, 5463, doi: 10.1016/j.stem.2009.05.003 (2009). Overall, many studies utilize IP injections to deliver therapeutics to lab animals due to the efficiency of the administration route. Article 107vs. 109 ; Fig. Intraperitoneal Versus Interpleural Morphine or Bupivacaine for Pain If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. -Lactalbumin is an example of a "retired" self-protein because its expression in normal . P values of<0.05 were considered statistically significant. [4] In 1964, a study delivered chemical agents such as acetic acid, bradykinin, and kaolin to mice intraperitoneally in order to study a "squirming" response. showed that incubation of human monoclonal IgG aggregates in plasma for 24 hrs resulted in alteration of the total number of aggregates, led to different aggregate size and changed their . They found that PCs can directly migrated from peritoneal cavity and some of them were found at intestinal villi. HHS Vulnerability Disclosure, Help By using this techniques, the catheter complications, in particular obstruction, bowel and vaginal cuff perforation were greatly reduced61. 2021 Dec;12(1):8635-8649. doi: 10.1080/21655979.2021.1988364. The immunohistochemistry indicated higher repair proliferation (Ki-67) and more FoxP3+ cells accumulation of IP group. Comparison of colonic FoxP3 expression 7 days after MSCs injection. The primer sequences used were listed in Supplementary Table S1. Radiation Protection of Polydatin Against Radon Exposure Injury of 2019 Jun 24;36(9):126. doi: 10.1007/s11095-019-2646-z.

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